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저칼슘식이와 방사선조사가 백서하악과두에 미치는 영향에 관한 실험적 연구
THE EFFECTS OF THE LOW CALCIUM DIET AND IRRADIATION ON THE MANDIBULAR CONDYLE OF RATS

치과방사선 1993년 23권 1호 p.27 ~ 48

안희문, 김태권,

소속 상세정보

안희문 ( ) - 경희대학교 치과대학 치과방사선학교실
김태권 ( ) - 경희대학교 치과대학 치과방사선학교실

KMID : 0378219930230010027

Abstract

골송조증으로 인한 악골의 변화상과 방사선이 골조직에 미치는 영향을 구명 하고자
0.01%의 칼슘함량식이를 7주령의 체중 150gm내외의 Sprague Dawley계 웅성백서에 투여하
여 골송조증을 유발시켰다. 이들 실험동물을 대상으로 정상식이군을 대조군으로, 저칼슘식이
군을 실험군으로 정한 후 대조군과 실험군을 각각 방사선조사군과 비조사군으로 다시 나누
었다. 대조군 및 실험군의 방사선조사군에는 10주령에 20 Gy의 Co-60을 단회조사하였으며,
각군의 실험동물을 방사선조사 3일, 7일, 14일, 21일후에 10두씩 희생시킨 다음 양측 하악과
두를 적출하여 dual energy X-ray absorptiometry와 soft X-ray로서 골밀도와 골소주 및
석회화대를 관찰하고, 병리조직학적 및 면역조직화학적 방법으로 검색하였던 바 다음과 같
은 결과를 얻었다.
1. 저칼슘식이 방사선비조사군에서는 골밀도가 실험 14일후부터는 현저히 감소되었으며,
골소주도 실험 3일후부터 이의 수가 감소되고 석회화대의 흡수소견도 관찰되었다. 병리조직
학적 검색에서는 실험 7일후부터 골소주의 수와 크기가 감소되는 소견이 보였다.
2. 저칼슘식이 방사선조사군에서는 골밀도가 실험 14일후부터 현저히 감소되었다. 또한 골
소주는 수가 감소되고 조조화되었으며, 석회화대의 흡수가 관찰되었는데, 이들의 변화상은
방사선비조사군에 비하여 실험 21일후에 가장 심하였다. 병리조직학적 검색에서는 실험 7일
이후에 다수의 파골세포가 관찰되었고, 골소주의 수와 크기도 다소 감소되었으며, 실험 14일
후에는 골수조직이 심하게 변성되다가 실험 21일후에 다소 회복되는 소견이 관찰되었다.
3. 정상식이 방사선조사군에서는 실험기간의 경과에 따라 골밀도가 근소하게 감소되었으
며, 골소주의 수의 감소 정도는 방사선 비조사군에 비하여 다소 컸다. 병리조직학적 검색에
서 는 실험 3일후부터 골수조직 은 위축, 변성되다가 실험 21일후에는 다소 회복된 소견이
관찰되었다.
4. 면역조직화학적 염색에서, 저칼슘식이의 방사선조사군과 비조사군은 Tumor Necrosis
Factor염색에 전반적으로 미약하거나 음성반응을 보였으나, 정상식이 방사선조사군과 비조
사군은 양성반응을 보였다.
#초록#
This study was performed to investigate the changes of mandibular condyle by low
calcium diet and the effects of irradiation on the bone in osteoporotic state.
In order to carry out this experiment, 80 seven-week old Sprague-Dawley strain rats
weighing about 150 gm were selected and equally divided into one experimental group
of 40 rats and one control group with the remainder. The experimental group and the
control group were then subdivided into two group and exposed to irradiation. The two
irradiation groups received a single dose of 20 Gy on the jaw area only and irradiated
with a cobalt-60 teletherapy unit. The rats in the control and experimental groups were
serially terminated by fours on the 3rd, the 7th, the 14th, and the 21s1 duty after
irradiation. After termination, both sides of the dead rats mandibular condyle were
removed and fixed with 10% neutral formalin. The bone mineral density of mandibular
condyle was measured by use of dual energy X-ray bone densitometer(model
DDX-alpha, Lunn Corp., U.S.A.). The mandibular condyle was radiographed with Hitex
HA-80(Hitex Co., Japan). Thereafter, the obtained radiographs were observed, and the
mandibular condyle was further decalcified and embedded in paraffin as the general
method. The specimen sectioned and stained with hematoxylin-eosin, PAS and Rabbit
Anti-Human Tumor Necrosis Factor-α observed by a light microscope.
The obtained results were as follows :
1. In the non-irradiated group with the low calcium diet, the bone mineral density of
the condyle was markedly decreased after 14 days, and decrease the number of
trabeculae of the condyle and resorption of the calcified cartilaginous 2one were
observed after 3 days. On microscopic observations, the number and size of trabeculae
were decreased after 7 days of experiment.
2. In the irradiated group with the low calcium diet, the bone mineral density of the
condyle was markedly decreased after 14 days and resorption of the calcified
cartilaginous zone and decrease the number and coarse of the trabeculae of the condyle
were observed. These findings were extended rather than in non-irradiated group with
low calcium diet. On microscopic observations, many osteoclasts were detested and the
number and size of trabeculae were somewhat decreased after 7 days. Also there was
degenerative changes of tissues of bone marrow on the 14th day but that condition was
restored on the 21st day of experiment.
3. In the irradiated group with normal diet, the bone mineral density of the condyle
was somewhat decreased with times and degree of decrease of the number of trabeculae
was somewhat larger than in the non-irradiated group with normal diet. On microscopic
observations, the tissues of bone marrow were atrophic and degenerative changes but
that condition was restored on the 21st day of experiment.
4. In immunocytochemical findings, in the irradiated and non-irradiated groups with
low calcium diet, negative or partial positive response to TNF was observed, but
positive response in the normal diet groups.